| LC-MS/MS内标法测定水产品中三聚氰胺残留量 |
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| 引用本文： | 孙伟红,冷凯良,邢丽红,王志杰,翟毓秀,王泽文.LC-MS/MS内标法测定水产品中三聚氰胺残留量[J].分析试验室,2009,28(10). |
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| 作者姓名： | 孙伟红 冷凯良 邢丽红 王志杰 翟毓秀 王泽文 |
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| 作者单位： | 1. 中国水产科学研究院黄海水产研究所,青岛,266071
2. 中国海洋大学,青岛,266100 |
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| 基金项目： | 农业部标准化项目和国家863计划 |
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| 摘 要： | 建立了高效液相色谱-电喷雾串联质谱(LC-MS/MS)测定水产品中残留的三聚氰胺的方法.以13C,15N标记三聚氰胺为内标,样品经三氯乙酸溶液沉淀蛋白,提取目标分析物,经SCX-C18均匀混合填装的反相色谱柱分离,选择反应监测(SRM)正离子模式测定.LC-MS/MS的检测低限为10 μg/kg;在10.0～500 ng/mL时峰强度与质量浓度的线性关系良好(r>0.99).方法的平均回收率为78%～118%.
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| 关 键 词： | 液相色谱串联质谱 内标法 水产品 三聚氰胺 |
Determination of melamine residue in aquatic products by high performance liquid chromatography-electrospray ionization tandem mass spectrometry with internal standard method |
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| SUN Wei-hong,LENG Kai-liang,XING Li-hong,WANG Zhi-jie,ZHAI Yu-xiu,WANG Ze-wen.Determination of melamine residue in aquatic products by high performance liquid chromatography-electrospray ionization tandem mass spectrometry with internal standard method[J].Chinese Journal of Analysis Laboratory,2009,28(10). |
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| Authors: | SUN Wei-hong LENG Kai-liang XING Li-hong WANG Zhi-jie ZHAI Yu-xiu WANG Ze-wen |
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| Institution: | 1.Yellow Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Qingdao 266071;2.Ocean University of China;Qingdao 266100 |
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| Abstract: | A method for the determination of melamine residue in aquatic products was developed using high performance liquid chromatography-electrospray ionization tandem mass spectrometry(LC-MS/MS).13C,15N-melamine used as an internal standard was added to the sample before extraction.Trichloroacetic acid solution was used to precipitate proteins and dissociate the target analyte from the sample matrix.A SCX-C18 mixture filled RP-HPLC column was used for separation,and the mass spectrometer was operated in the posit... |
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| Keywords: | High performance liquid chromatography-electrospray ionization tandem mass spectrometry Internal standard method Aquatic products Melamine |
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