金属配位化合物在生物分析、成像以及染色方面的应用

由于优越的光物理和光化学特性,很多金属配合物长期以来被用于生物传感器、生物探针和医学诊断等领域。本文综述了近年来已经或有望成功应用于生命科学基础研究和临床医学诊断众多领域中的多种金属配位化合物,包括几种在成功商业化的体外诊断系统中作为分子探针、生物标记物或蛋白染色剂而广泛应用的金属钌配位化合物和稀土金属配位化合物,在核磁共振成像技术中作为造影剂或影像增强剂的系列金属钆配位化合物,以及在细胞成像、生物标记和蛋白质分析应用中极具商业化应用前景的一些新型环金属铱配位化合物等。此外,本文还对金属配合物结合纳米结构及技术在生物医学领域中的应用做了简单介绍和展望。

Metal Complexes and Their Applications in Bioassay, Bio-Imaging and Protein Staining

Metal complexes, which possess unique photophysical and photochemical properties, have played important roles in many fields of life sciences and medical diagnostics. The rapidly expanding family of metal complexes, with large Stokes-shifts, multi-color tunability and stability against photobleaching, represents an attractive resource to chemists and life scientists seeking novel bio-labeling and bio-imaging methodologies. In this mini-review, we describe several well established bioanalytical and in vitro diagnostic systems that utilize ruthenium and rare earth metal complexes as the luminescent labeling molecules, gadolinium complexes as contrast agents in magnetic resonance imaging and novel iridium complexes as cell staining or protein staining agents. In addition, the construction of nano-architecture and combination with nanotechnology for biomedical application of potential metal complexes are also briefly introduced. Contents
1 Introduction
2 Metal complexes used in commercial technologies
2.1 Ru(bpy)₃²⁺ and other ruthenium complexes containing N^N bidentate ligands
2.2 Luminescent rare earth metal complexes
2.3 Contrast agents for magnetic resonance imaging
3 Iridium complexes and their potential applications
3.1 Cellular imaging
3.2 Bio-labeling
3.3 Protein assay
4 Metal complex nano-architectures for bioassay
5 Summary and prospects