荧光纳米颗粒标记免疫分析*

荧光纳米颗粒标记是目前免疫分析研究中一个新兴的领域。由于荧光纳米颗粒标记可以有效地提高单个识别分子上标记的荧光量（F/P），从而大大提高分析的灵敏度。但是由于在标记和免疫分析过程中具有很多不同于传统荧光染料的特点，需要很多经验的积累，在现阶段限制了其在实际医学诊断中的广泛应用。本文就近年来荧光纳米颗粒标记免疫分析中涉及到的标记方法、免疫模式、影响因素等方面进行了综述。

Fluorescent Nanoparticle Labeled Immunoassay

Fluorescent nanoparticles are popular labels in immunoassay research. They effectively increase the number of fluorescers on a detection molecule and contribute to ultra high sensitivity. But there are many differences in labeling and immunoassay of nanoparticles from conventional fluorescent labels. This restrains their broad application in diagnosis. The labeling methods, immunological configurations and influence factors of fluorescent nanoparticles in recent years are reviewed.