Development and Optimisation of HILIC-LC-MS Method for Determination of Carbohydrates in Fermentation Samples |
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| Authors: | Dmitri Pismenni Vassili Kiritsenko Jaroslav Marhivka Mary-Liis Kütt Raivo Vilu |
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| Institution: | 1.Center of Food and Fermentation Technologies (TFTAK), Akadeemia tee 15A, 12618 Tallinn, Estonia; (V.K.); (J.M.); (M.-L.K.); (R.V.);2.Department of Chemistry and Biotechnology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia |
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| Abstract: | Saccharides are the most common carbon source for Streptococcus thermophilus, which is a widely used bacterium in the production of fermented dairy products. The performance of the strain is influenced by the consumption of different saccharides during fermentation. Therefore, a precise measurement of the concentrations of saccharides in the fermentation media is essential. An 18-min long method with limits of quantitation in the range of 0.159–0.704 mg/L and with 13C labelled internal standards employing hydrophilic interaction chromatography coupled to mass spectrometric detection-(HILIC-LC-MS) allowed for simultaneous quantification of five saccharides: fructose, glucose, galactose, sucrose, and lactose in the fermentation samples. The method included a four-step sample preparation protocol, which could be easily applied to high-throughput analysis. The developed method was validated and applied to the fermentation samples produced by Streptococcus thermophilus. |
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| Keywords: | saccharides HILIC-LC-MS Streptococcus thermophilus fermentation |
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