Solvent Mixture Optimization in the Extraction of Bioactive Compounds and Antioxidant Activities from Garlic (Allium sativum L.) |
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Authors: | Vytria Piscitelli Cavalcanti Smail Aazza Suzan Kelly Vilela Bertolucci Joo Pedro Miranda Rocha Adriane Duarte Coelho Altino Júnior Mendes Oliveira Laís Campelo Mendes Maysa Mathias Alves Pereira Ludmila Caproni Morais Moacir Rossi Forim Moacir Pasqual Joyce Dria |
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Institution: | 1.Department of Agriculture, Federal University of Lavras (UFLA), Lavras 37200-900, Brazil; (V.P.C.); (S.K.V.B.); (J.P.M.R.); (A.D.C.); (A.J.M.O.); (L.C.M.); (M.M.A.P.); (L.C.M.); (M.P.);2.Laboratory of Phytochemistry, National Agency of Medicinal and Aromatic Plants (NAMAP/ANPMA), Taounate 34202, Morocco;3.Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos 13565-905, Brazil; |
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Abstract: | Garlic is a health promoter that has important bioactive compounds. The bioactive extraction is an important step in the analysis of constituents present in plant preparations. The purpose of this study is to optimize the extraction with the best proportion of solvents to obtain total phenolic compounds (TPC) and thiosulfinates (TS) from dried garlic powder, and evaluate the antioxidant activities of the optimized extracts. A statistical mixture simplex axial design was used to evaluate the effect of solvents (water, ethanol, and acetone), as well as mixtures of these solvents, after two ultrasound extraction cycles of 15 min. Results showed that solvent mixtures with a high portion of water and pure water were efficient for TPC and TS recovery through this extraction procedure. According to the regression model computed, the most significant solvent mixtures to obtain high TPC and TS recovery from dried garlic powder are, respectively, the binary mixture with 75% water and 25% acetone and pure water. These optimized extracts presented oxygen radical absorbance capacity. Pure water was better for total antioxidant capacity, and the binary mixture of water–acetone (75:25) was better for DPPH scavenging activity. These optimized extracts can be used for industrial and research applications. |
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Keywords: | extraction optimization solvent mixtures response surface methodology phenols thiosulfinates antioxidants |
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