A single amino acid Gly-tag enables metal-free protein purification |
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| Authors: | Landa Purushottam Unnikrishnan V B Dattatraya Gautam Rawale Mansi Gujrati Surya Dev Mishra Sajeev T K Neelesh C Reddy Srinivasa Rao Adusumalli Ram Kumar Mishra Vishal Rai |
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| Institution: | Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhopal Bypass Road, Bhauri, Bhopal 462 066 India.; Department of Biological Sciences, Indian Institute of Science Education and Research Bhopal, Bhopal Bypass Road, Bhauri, Bhopal 462 066 India |
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| Abstract: | Analytically pure proteins are indispensable for diverse applications, including therapeutics. Here, we report a methodology where a single amino acid, glycine, enables metal-free protein purification. This robust platform is enabled by a Gly-tag resin for site-specific capture, enrichment, and release through chemically triggered C–C bond dissociation by resonance-assisted electron density polarization.Gly-tag resin precisely captures and releases a protein with one glycine at the N-terminus. The user-friendly protocol delivers analytically pure protein free of metal contaminants. |
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