Surface plasmon resonance |
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Authors: | Priyabrata Pattnaik |
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Institution: | (1) Virology Division, Defence Research and Development Establishment, Jhansi Road, 474 002 Gwalior, India |
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Abstract: | During last decade there has been significant progress in the development of analytical techniques for evaluation of receptor-ligand
iteraction. Surface plasmon resonance (SPR)-based optical biosensors are now being used extensively to defined the kinetics
of wide variety of macromolecular interactions and high- and low-affinity small molecule interactions. The experimental design
data analysis methods are evolving along with widespread applications in ligand fishing, microbiology, virology, host-pathogen
interaction, epitope mapping and protein-, cell-, membrane-, nucleic acid-protein interactions. SPR based biosensors have
strong impact on basic and applied research significantly. This brief review describes the SPR technology and few of its applications
in relation to receptor-ligand interaction that has brought significant change in the methodology, analysis, interpretation,
and application of the SPR technology. |
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Keywords: | Surface plasmon resonance (SPR) optical biosensor receptor-ligand interaction Biacore ligand fishing |
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