Study on methane fermentation and production of vitamin B12 from alcohol waste slurry |
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| Authors: | Zhenya Zhang Taisheng Quan Pomin Li Yansheng Zhang Norio Sugiura Takaaki Maekawa |
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| Institution: | (1) Institute of Agricultural and Forest Engineering, University of Tsukuba, 1-1-1 Tennodai, 305-8573 Tsukuba, Ibaraki, Japan;(2) Mater Program of Biosystem Studies, University of Tsukuba, 1-1-1 Tennodai, 305-8573 Tsukuba, Ibaraki, Japan;(3) Doctoral Program of Agricultural Science, University of Tsukuba, 1-1-1 Tennodai, 305-8573 Tsukuba, Ibaraki, Japan;(4) Water Conservancy and Civil Engineering College, China Agricultural University, Peking, Haidian District, Qinhua Dong Road, China |
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| Abstract: | We studied biogas fermentation from alcohol waste fluid to evaluate the anaerobic digestion process and the production of
vitamin B12 as a byproduct. Anaerobic digestion using acclimated methanogens was performed using the continuously stirred tank reactor
(CSTR) and fixed-bed reactor packed with rock wool as carrier material at 55°C. We also studied the effects of metal ions
added to the culture broth on methane and vitamin B12 formation. Vitamin B12 production was 2.92 mg/L in the broth of the fixed-bed reactor, twice that of the CSTR. The optimum concentrations of trace
metal ions added to the culture liquid for methane and vitamin B12 production were 1.0 and 8 mL/L for the CSTR and fixed-bed reactor, respectively. Furthermore, an effective method for extracting
and purifying vitamin B12 from digested fluid was developed. |
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| Keywords: | Vitamin B12 methane acclimated methanogens trace metal ions rock wool |
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