Evaluation of recombinant strains of Zymomonas mobilis for ethanol production from glucose/xylose media |
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Authors: | Eva Joachimsthal Kevin D Haggett Peter L Rogers |
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Institution: | (1) Department of Biotechnology, University of New South Wales, 2052 Sydney, Australia |
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Abstract: | The fermentation characteristics of two recombinant strains of Zymomonas mobilis, viz. CP4 (pZB5) and ZM4 (pZB5), capable of converting both glucose and xylose to ethanol, have been characterized in batch
and continuous culture studies. The strain ZM4 (pZB5) was found to be capable of converting a mixture of 65 g/L glucose and
65 g/L xylose to 62 g/L ethanol in 48h with a yield of 0.46 g/g. Higher sugar concentrations resulted in incompletexylose
utilization (80h) presumably owing to ethanol inhibition of xylose assimilation or metabolism. The fermentation results with
ZM4 (pZB5) show a significant improvement over results published previously for recombinant yeasts and other bacteria capable
of glucose and xylose utilization. |
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Keywords: | Recombinant Zymomonas mobilis xylose fermentation lignocellulosic hydrolysates ethanol production |
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