Reasons for the high stability of fumarase activity ofBrevibacterium flavum cells immobilized with k-carrageenan gel |
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Authors: | Isao Takata Tetsuya Tosa Ichiro Chibata |
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Institution: | (1) Department of Biochemistry, Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd., 16-89, Kashima-3-chome, Yodogawa-ku, Osaka, Japan |
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Abstract: | Whole cells ofBrevibacterium flavum having high fumarase activity were immobilized using K-carrageenan. The reason for the high stability of fumarase activity
of immobilized cells was investigated.
One of main reasons for stabilizing fumarase activity by immobilization using K-carrageenan against organic solvents such
as ethanol and acetone was the lower concentration of these solvents in the carrageenan gel compared with that in outer bulk
solution. The stabilization of fumarase activity in the immobilized cells against protein-denaturing reagents was found to
be related to rheological properties of K-carrageenan gel. Another reason for stabilizing fumarase activity by immobilization
with K-carrageenan was to protect the cells from lysis.
When immobilized cells were freeze-thawed, their fumarase activity increased and operation stability decreased. Therefore,
one reason for the high decay of fumarase activity caused by the freeze-thawing may be a change in the pore size of the K-carrageenan
gel.
Fumarase activity and the operational stability of immobilized cells was found to depend on gelling conditions. Therefore,
the steric structure of the K-carrageenan gel may be related to the decay of fumarase activity. |
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Keywords: | K-Carrageenan immobilized cells ofBrevibacterium flavum fumarase activity in immobilized cells stabilization of enzyme by immobilization with K-carrageenan Brevibacterium flavum immobilized |
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