A high-copy-number plasmid capable of replication in thermophilic cyanobacteria |
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| Authors: | Masato Miyake Hiroshi Nagai Makoto Shirai Ryuichiro Kurane Yasuo Asada |
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| Institution: | (1) National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Ministry of International Trade and Industry, Higashi 1-1, 305-8566 Tsukuba, Ibaraki, Japan;(2) Division of Biotechnology, Ibaraki University, Ibaraki, Japan |
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| Abstract: | A 2.5 kb high-copy-number plasmid, pM A4 in thermophilic cyanobacterium Synechococcus sp. M A4 was isolated and characterized to develop a genetic engineering system for thermophilic cyanobacteria. The copy
number of pM A4 was determined to be by densitometry about 350/cell. The pM A4 may be a type of rolling-circle plasmid, because
a possible rep gene encoding 34 k D-protein and a consensus sequence of a double-stranded origin nick site of rolling circle plasmids were
found in the pM A4 sequence. The pM A4 was electro-introduced into another thermophile, Synechococcus sp. MA 19, which is the strongest poly-β-hydroxybutyrate (PHB) accumulator in photoau totrophic organisms. The pM A4 was
incorporated and retained in MA 19. These results indicate that pM A4 could be developed as a useful vector for thermophilic
cyanobacteria. |
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| Keywords: | Thermophilic cyanobacteria poly-β -hydroxybutyrate carbon dioxide fixation biodegradable plastic genetic engineering rolling circle plasmid |
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