Antioxidant Activity and Protective effect of Banana Peel against Oxidative Hemolysis of Human Erythrocyte at Different Stages of Ripening |
| |
Authors: | Shanthy Sundaram Shadma Anjum Priyanka Dwivedi Gyanendra Kumar Rai |
| |
Institution: | (1) Centre for Biotechnology, University of Allahabad, Allahabad, 211002, India;(2) Centre of Food Technology, University of Allahabad, Allahabad, 211002, India |
| |
Abstract: | Phytochemicals such as polyphenols and carotenoids are gaining importance because of their contribution to human health and
their multiple biological effects such as antioxidant, antimutagenic, anticarcinogenic, and cytoprotective activities and
their therapeutic properties. Banana peel is a major by-product in pulp industry and it contains various bioactive compounds
like polyphenols, carotenoids, and others. In the present study, effect of ripening, solvent polarity on the content of bioactive
compounds of crude banana peel and the protective effect of peel extracts of unripe, ripe, and leaky ripe banana fruit on
hydrogen peroxide-induced hemolysis and their antioxidant capacity were investigated. Banana (Musa paradisica) peel at different stages of ripening (unripe, ripe, leaky ripe) were treated with 70% acetone, which were partitioned in
order of polarity with water, ethyl acetate, chloroform (CHCl3), and hexane sequentially. The antioxidant activity of the samples was evaluated by the red cell hemolysis assay, free radical
scavenging (1,1-diphenyl-2-picrylhydrazyl free radical elimination) and superoxide dismutase activities. The Folin–Ciocalteu's
reagent assay was used to estimate the phenolic content of extracts. The findings of this investigation suggest that the unripe
banana peel sample had higher antioxidant potency than ripe and leaky ripe. Further on fractionation, ethyl acetate and water
soluble fractions of unripe peel displayed high antioxidant activity than CHCl3 and hexane fraction, respectively. A positive correlation between free radical scavenging capacity and the content of phenolic
compound were found in unripe, ripe, and leaky ripe stages of banana peel. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|