3-Isopropyloxy-6-morpholino-2-phenylphenalen-1-one as Lipophilic Fluorescent Probe for Lymphocyte Investigations |
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Authors: | Elena M Kirilova Inta Kalnina |
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Institution: | (1) Daugavpils University, Daugavpils, Latvia |
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Abstract: | Fluorescent properties of two naphthalimides and a phenalenone derivative in organic solvents and when they bind to human
peripheral blood lymphocytes were investigated. Different spectral characteristics were observed using lymphocytes of healthy
donors and patients with nonmalignant (chronic myeloid leukemia) and malignant (B-cell lymphoid leukemia) diseases. It was
found that spectral properties of the used fluorophores in cell suspension qualitatively characterize its structural and functional
alterations during pathological phenomena. The intensity of fluorescence increased in samples from patients with B-cell lymphoid
leukemia, and the fluorescence maximum shifted to the long-wavelength region by 20 nm compared with normal lymphocytes. It
is concluded that 3-isopropyloxy-6-morpholino-2-phenylphenalen-1-one as most promising probe may be applied to the study of
malignant diseases. |
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