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Biotechnological Utilization of Biodiesel-Derived Glycerol for the Production of Ribonucleotides and Microbial Biomass |
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| Authors: | Juan Daniel Rivaldi Boutros Fouad Sarrouh Ricardo de Freitas Branco Ismael Maciel de Mancilha Silvio Silvério da Silva |
| Institution: | 1. Departamento de Biotecnologia??LOT, Escola de Engenharia de Lorena, Universidade de S?o Paulo, Estrada Municipal do Campinho, PO Box 116, 12602810, Lorena, S?o Paulo, Brazil 2. Facultad de Ciencias Qu??micas, Universidad Nacional de Asunci??n, Ruta Mcal Estigarriba, Km 11 C.P. 1055, San Lorenzo, Paraguay 3. Coordena??o de Qu??mica, Universidade Tecnol??gica Federal do Paran????Campus Pato Branco, Via do Conhecimento, Km 1, CEP 85503-390, Pato Branco, Paran??, Brazil |
| Abstract: | Ten yeast strains were evaluated concerning their capabilities to assimilate biodiesel-derived glycerol in batch cultivation. The influence of glycerol concentration, temperature, pH and yeast extract concentration on biomass production was studied for the yeast selected. Further, the effect of agitation on glycerol utilization by the yeast Hansenula anomala was also studied. The yeast H. anomala CCT 2648 showed the highest biomass yield (0.30?g?g?1) and productivity (0.19?g?L?1?h?1). Citric acid, succinic acid, acetic acid and ethanol were found as the main metabolites produced. The increase of yeast extract concentration from 1 to 3?g?L?1 resulted in high biomass production. The highest biomass concentration (21?g?L?1), yield (0.45?g?g?1) and productivity (0.31?g?L?1?h?1), as well as ribonucleotide production (13.13?mg?g?1), were observed at 700?rpm and 0.5?vvm. These results demonstrated that glycerol from biodiesel production process showed to be a feasible substrate for producing biomass and ribonucleotides by yeast species. |
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