Cellulase-Producing Bacteria from Thai Higher Termites, <Emphasis Type="Italic">Microcerotermes</Emphasis> sp.: Enzymatic Activities and Ionic Liquid Tolerance |
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| Authors: | Kitipong Taechapoempol Thammanoon Sreethawong Pramoch Rangsunvigit Weerachart Namprohm Bandhit Thamprajamchit Sirirat Rengpipat Sumaeth Chavadej |
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| Institution: | (1) The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok, 10330, Thailand;(2) Center for Petroleum, Petrochemicals, and Advanced Materials, Chulalongkorn University, Bangkok, 10330, Thailand;(3) Thaioil Public Company Limited, Tungsukla, Sriracha, Cholburi, 20230, Thailand;(4) Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; |
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| Abstract: | The three highest hydrolysis-capacity-value isolates of Bacillus subtilis (A 002, M 015, and F 018) obtained from Thai higher termites, Microcerotermes sp., under different isolation conditions (aerobic, anaerobic, and anaerobic/aerobic) were tested for cellulase activities—FPase,
endoglucanase, and β-glucosidase—at 37 °C and pH 7.2 for 24 h. Their tolerance to an ionic liquid, 1-butyl-3-methylimidazolium
chloride (BMIM]Cl), was also investigated. The results showed that the isolate M 015 provided the highest endoglucanase activity
whereas the highest FPase and β-glucosidase activities were observed for the isolate F 018. The isolate F 018 also showed
the highest tolerance to BMIM]Cl in the range of 0.1–1.0 vol.%. In contrast, the isolate A 002 exhibited growth retardation
in the presence of 0.5–1.0 vol.% BMIM]Cl. |
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