Polyhydroxybutyrate production from carbon dioxide by cyanobacteria |
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| Authors: | Miyake Masato Takase Kazuya Narato Midori Khatipov Emir Schnackenberg Joerg Shirai Makoto Kurane Ryuichiro Asada Yasuo |
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| Institution: | (1) National Institute of Bioscience and Human-Technology, Higashi 1-1, Tsukuba, 305-8566 Ibaraki, Japan;(2) Division of Biotechnology, Ibaraki University, Ami, Inashiki, 300-03 Ibaraki, Japan;(3) Department of Molecular Genetics and Cell Biology, Cummings Life Science Center, University of Chicago, 920 East 58th Street, 60637 Chicago, IL;(4) Industrial Technology Center of Okayama Prefecture, 5301 Haga, 701-1296 Okayama, Japan |
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| Abstract: | Genetic characterization and enhancement of polyhydroxybutyrate (PHB) accumulation in cyanobacteria were investigated for
efficient PHB production from CO2. The genome DNAs in the PHB-accumulating strains Synechococcus sp. MA19 and Spirulina platensis NIES46 retained the highly homologous region to phaC of Synechocystis PCC6803, whereas low homology was detected in the nonaccumulating strains Synechococcus sp. PCC7942 and Anabaenacylindrica NIES19. Synechococcus sp. MA19, which accumulates PHB up to 30% of dry cell weight from CO2 as the sole carbon source, was mutated by insertion of transposon Tn5 to enhance the PHB accumulation. Genetic and physiological
analysis of the mutant indicated that decreased phosphotransacetylase activity could trigger an increase of acetyl coenzyme
A leading to enhancement of PHB accumulation. PHB synthase in Synechococcus sp. MA19 was probably attached to thylakoid membrane since PHB granules were associated with pigments. A genetically engineered
cyanobacteria retaining soluble PHB synthase from Ralstonia eutropha accumulated pigment-free PHB granules, which is an advantage for the purification of PHB. |
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| Keywords: | Polyhydroxyalkanoates cyanobacteria carbon dioxide |
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