Xylanolytic enzyme production by anAspergillus niger isolate |
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Authors: | M Costa-Ferreira A Dias C Maximo M J Morgado G Sena-Martins J Cardoso Duarte |
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Institution: | (1) Biotechnology Unit, National Institute, for Industrial Engineering and Technology, INETI/DTIQ, Est. das Palmeiras, 2745 Queluz de Baixo, Portugal |
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Abstract: | Production of xylanolytic enzymes by anAspergillus niger CCMI 850 isolate was investigated in batch cultures. The effect of the composition of a fermentation medium that did not
include chemical inducers, on β-xylanase, β-xylosidase, α-l-arabinofuranosidase, and total cellulase activity was studied. With 4% xylan as the carbon source, about 65 U/mL of β-xylanase
was obtained, whereas the total cellulase activity was undetectable, under the specified conditions. This β-xylanase activity
represents the highest reported for a wild-type strain ofA. niger. The effect of pH and temperature on the activity of β-xylanase was studied. Partial characterization of the β-xylanase showed
that with insoluble birchwood as substrate theK
m
andV
max were 0.3 mM and 19 μmol/min, respectively. Aspects of using the crude β-xylanase preparation for applications in the pulp and paper industry
were discussed. |
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Keywords: | Aspergillus niger xylanase β -xylosidase low cellulase production pulp and paper industry applications |
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