Culture conditions dictate protease and tannase production in submerged and solid-state cultures of Aspergillus niger Aa-20 |
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Authors: | Aguilar Cristóbal Noé Favela-Torres Ernesto Vinegra-González Gustavo Augur Christopher |
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Institution: | (1) Food Research Department, Faculty of Chemical Sciences, Universidad Autónoma de Coahuila, Unidad Saltillo, Coah., PO Box 252, 25 000 México;(2) Biotechnology Department, Universidad Autónoma Metropolitana, Unidad Iztapalapa, PO Box 55-535, 09340 México, D.F.;(3) Institut de Recherche pour le Développement, IRD-France CESB/ESIL, 163 Avenue de Luminy F, 13288 Marseille, Cedex 9, France;(4) Present address: The University of Georgia, Complex Carbohydrate Research Center, 220 Riverbend Road, 30602-4712 Athens, GA, USA |
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Abstract: | Undesirable protease production by Aspergillus niger Aa-20 in submerged culture and solid-state culture was evaluated using different concentrations of tannic acid as sole carbon
source in a model system designed for tannase production. Protease production was found to be dependent on the culture system
used (submerged culture or solid-state culture) and on the initial tannic acid concentration. Expression of protease activity
in submerged culture was higher (up to 10 times) than activity obtained in solid-state culture, using identical culture medium
composition. In submerged culture, the lowest final protease activity (0.13 IU) was obtained with the highest tannic acid
concentration, while in solid-state culture protease activity was not affected by changes in initial substrate concentration.
Absence of detectable proteolytic activity in solid-state culture is related to high production of tannase enzyme. Hence,
the use of solid-state culture for fungal enzyme production may allow for higher and more stable enzyme titers present in
culture extracts. |
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Keywords: | Protease solid-state fermentation tannase tannin acyl hydrolase liquid fermentation submerged fermentation Aspergillus niger tannic acid |
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