Utilization of Horticultural Waste for Laccase Production by <Emphasis Type="Italic">Trametes versicolor</Emphasis> Under Solid-State Fermentation |
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Authors: | Fengxue?Xin Email author" target="_blank">Anli?GengEmail author |
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Institution: | (1) School of Life Sciences and Chemical Technology, Ngee Ann Polytechnic, 535 Clementi Road, Singapore, 599489, Singapore; |
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Abstract: | Horticultural waste collected from a landscape company in Singapore was utilized as the substrate for the production of laccase
under solid-state fermentation by Trametes versicolor. The effects of substrate particle size, types of inducers, incubation temperature and time, initial medium pH value, and
moisture content on laccase production were investigated. The optimum productivity of laccase (8.6 U/g substrate) was achieved
by employing horticultural waste of particle size greater than 500 μm and using veratryl alcohol as the inducer. The culture
was at 30 °C for 7 days at moisture content of solid substrate of 85% and initial pH 7.0. The decolorization was also investigated
in order to assess the degrading capability of the ligninolytic laccase obtained in the above-mentioned cultures. The decolorization
degree of a model dye, phenol red, was around 41.79% in 72 h of incubation. By far, this is the first report on the optimization
of laccase production by T. versicolor under solid-state fermentation using horticultural waste as the substrate. |
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Keywords: | |
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