High-performance liquid chromatography and supercritical fluid chromatography of monosaccharides and polyols using light-scattering detection Chemometric studies of the retentions

Twelve sugars and polyols were analyzed using high-performance liquid chromatographic (HPLC) and supercritical fluid chromatographic (SFC) systems with silica and bonded silica stationary phases with the help of an evaporative light-scattering detector. The separation capacities of the two techniques are discussed. The retention data were studied using different chemometric methods (automatic classification, factor analysis). They clearly show that SFC and HPLC have the same retention process for these compounds and that is the sum of only two mathematically independent physico-chemical phenomena.