Aptamer-targeted magnetic nanospheres as a solid-phase extraction sorbent for determination of ochratoxin A in food samples |
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Authors: | Wu Ximei Hu Jun Zhu Binghui Lu Lin Huang Xiangdong Pang Daiwen |
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Institution: | College of Chemistry and Molecular Sciences, Wuhan University, Luojiashan, Wuhan, China. wxmdwb927@126.com |
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Abstract: | A sorbent based on the aptamer for ochratoxin A was immobilized onto magnetic nanospheres (MNS) and used to develop a magnetic solid-phase extraction procedure to clean up food samples in conjunction with high-performance liquid chromatography separation and fluorescence detection. Specific retention of ochratoxin A by the sorbent was demonstrated, and the capacity of the MNS-aptamer sorbent was determined. The efficacy of this new approach was successfully evaluated through comparison with solid-phase extraction on commercial C18 cartridge. Several different food samples fortified in the range of with 2.5-50 μg/kg yielded mean recoveries from 67% to 90%, respectively. Finally, this oligosorbent was applied to the selective extraction of ochratoxin A from unfortified food samples. |
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Keywords: | Ochratoxin A Aptamer Magnetic nanospheres Solid-phase extraction Food analysis |
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