Rapid and sensitive method for the determination of albendazole and albendazole sulphoxide in biological fluids |
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| Authors: | P E Hoaksey K Awadzi S A Ward P A Coventry M L Orme G Edwards |
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| Institution: | Department of Pharmacology and Therapeutics, University of Liverpool, U.K. |
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| Abstract: | A sensitive and selective reversed-phase high-performance liquid chromatographic method for the determination of albendazole and its active metabolite albendazole sulphoxide in plasma has been developed. It involves single-step extraction of plasma with dichloromethane, evaporation of the solvent and chromatography on a muBondapak phenyl column with a mobile phase of water containing 1% (v/v) triethylamine-methanol-acetonitrile (70:10:20, v/v) at pH 3.1. Run time is 12 min. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies and possesses important advantages, notably speed and expense, over current methods. |
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