Determination of abamectin residues in fruits and vegetables by high-performance liquid chromatography |
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Authors: | Diserens H Henzelin M |
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Institution: | NESTEC Ltd., Nestlé Research Centre, Lausanne, Switzerland. |
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Abstract: | A rapid and sensitive HPLC method has been developed and validated for the determination of abamectin residues (avermectin B1a and B1b, as well as the metabolite 8,9-Z-avermectin B1) in apples, pears and tomatoes. Residues are extracted with acetonitrile. The diluted extract is cleaned up on a C18 solid-phase extraction cartridge. Abamectin residues are derivatised with trifluoroacetic acid and 1-methylimidazole and determined by reversed-phase liquid chromatography with fluorescence detection (excitation: 365 nm and emission: 470 nm). High and consistent recoveries, ranging from 88 to 106%, were obtained, at spiking levels of 10, 20 and 50 micrograms/kg, when analysing apples, pears and tomatoes. |
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