Ultra-High-Pressure Liquid Chromatographic method for the analysis of tocopherols in human colostrum and milk |
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Authors: | Carolina Moltó-Puigmartí Ana Isabel Castellote M Carmen López-Sabater |
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Institution: | 1. Dept. of Nutrition and Food Science, Faculty of Pharmacy, University of Barcelona, Av. Joan XXIII s/n, E-08028 Barcelona, Spain;2. CIBER Epidemiología y Salud Pública (CIBERESP) Barcelona, Spain |
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Abstract: | A rapid, highly sensitive and direct Ultra-High-Pressure Liquid Chromatographic method was developed and validated for quantifying δ-, β + γ-, and α-tocopherol in human colostrum and milk. Two reversed-phase chromatographic columns and two detectors (Fluorescence Detector or FD and Photodiode Detector Array or PDA) were used and both methods were independently validated. Two internal standards were selected according to the detector used. Recoveries ranged from 96.71% to 103.55% and the relative standard deviations for the within-day precision were below 6% (PDA) and 3% (FD). Both approaches enabled to achieve low detection limits, on the order of ng (PDA) or pg (FD). Only 300 μL of sample and a chromatographic run of less than 1.6 min were enough to efficiently quantify the isomers in the colostrum and milk of Spanish women. |
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Keywords: | Tocopherols Antioxidants Human milk Colostrum Ultra-High-Pressure Liquid Chromatography (UHPLC)&ndash PDA&ndash FD Vitamin E |
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