| Abstract: | The characterisation of two cross-linked poly(acryloylmorpholines), Enzacry Gel K1 and Enzacryl Gel K2, as matrices for aqueous gel permeation chromatography is described. Near ideal plots of logarithm molecular weight versus distribution coefficient, Kd, are obtained for polyethylene glycols and linear, oligomeric α,ω-diols approximate molecular weight (
n) fractionation ranges being 0–4000 and 0–20,000 for Enzacryl Gel K1 and Enzacryl Gel K2, respectively. Anomalous retardation of the Schardinger dextrins, cyclomaltohexose and cyclomaltoheptose, is observed although linear maltosaccharides behave normally. The internal gel volumes, calculated from column elution data in water, are significantly larger than the volumes of solvent imbibed by the dry column packings on constituting the gel. Internal gel volumes and solvent imbibition volumes in water are compared with the corresponding values obtained in chloroform and tetrahydrofuran. The two parameters are discussed, in the case of Enzacryl Gel K2 in water and chloroform, in the light of plots of logarithm viscometric hydrodynamic volume versus Kd for polyethylene glycols. |
