Institution: | Istituto di Chimica, Università di Udine, Via Cotonificio 108, 33100 Udine Italy Dipartimento di Economia e Merceologia delle Risorse Naturalie delta Produzione, Università di Trieste, Via Valerio 6, 34127 Trieste Italy Laboratorio Chimico Compartimentale delle Dogane di Venezia, Via Ca' Marcello 15, 30172 Mestre Italy Istituto di Chimica, Università di Udine, Via Cotonificio 108, 33100 Udine Italy |
Abstract: | Official methods for the determination of 2-furaldehyde in spirits involve for a spectrophotometric evaluation, which is characterized by poor specificity. Gas chromatographic evaluations have also been proposed, which offer a much higher sensitivity, particularly when capillary columns are used. In this paper a high-performance liquid chromatographic (HPLC) method based on the formation of the 2,4-dinitrophenylhydrazones of carbonyl compounds and subsequent reversed-phase separation of these derivatives is described. Derivatization is carried out by utilizing an acidic solution of 2,4-dinitrophenylhydrazine in acetonitrile. Precipitation of the derivatives is avoided, and direct injection of the sample into the HPLC system is allowed. The determination ofrers a high specificity and a detection limit of the order of 10−8 mol/1. Accuracy and reproducibility data are presented. |