Intermittent counter-current extraction as an alternative approach to purification of Chinese herbal medicine |
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Authors: | Peter Hewitson Svetlana Ignatova Haoyu Ye Lijuan Chen Ian Sutherland |
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Institution: | 1. Advanced Bioprocessing Centre, Brunel Institute for Bioengineering, Brunel University, Uxbridge UB8 3PH, UK;2. State Key Laboratory of Biotherapy, West China Hospital and College of Chemical Engineering, Sichuan University, Chengdu 610041, China |
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Abstract: | This paper describes intermittent counter-current extraction, a novel method of using a conventional twin column counter-current chromatograph to either split a sample into two groups of compounds or extract and enrich a target compound from a crude extract. The first method is demonstrated by splitting a model mixture of four compounds into two groups. The second method is demonstrated by the extraction and enrichment of a high value target compound, triptolide, from a Chinese herbal medicine crude extract of Tripterygium wilfordii Hook. f., where it is found at low concentration (2%). This was achieved by retaining and enriching the target compound within the column while washing away all other components of the crude material. The success of the first method allowed the second method to be carried out without the need for costly preliminary experiments with the high value sample. 188 mg of triptolide at greater than 98% purity was separated from 9.2 g of crude extract, using 10 l of solvent in a 3-h separation. |
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Keywords: | True moving bed TMB Enrichment Chinese herbal medicine Pharmaceutical Tripterygium wilfordii Hook f Triptolide Intermittent counter-current extraction ICcE |
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