Facile analysis of short-chain fatty acids as 4-nitrophenyl esters in complex anaerobic fermentation samples by high performance liquid chromatography |
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Authors: | Schiffels Johannes Baumann Marcus E M Selmer Thorsten |
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Institution: | FH Aachen, Institut für Nano- und Biotechnologien, Heinrich-Mussmann-Str. 1, 52428 Jülich, Germany. |
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Abstract: | Short-chain fatty acids are crucial intermediates in the conversion of biomass to methane. Due to the complexity of raw biomass, volatile fatty acids (including n- and branched-chain compounds) as well as arylacetic and arylpropionic acids arise from digestion of carbohydrates, proteins and lipids. The development of a simple extraction procedure in combination with internal standardization and facile 4-nitrophenyl-labelling via oxalylchloride-generated acylchlorides enabled robust separation and quantification of the target compounds in crude biological samples like raw cattle manure and biogas fermenter contents. Detection limits of <100 μM and error rates of less than 4% for the quantification of individual compounds in a concentration range up to 50 mM for non-diluted samples suggest that the novel method might be of general advantage for the routine quantification of short-chain fatty acids in complex biological samples including complex fermentation media. |
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Keywords: | Short-chain fatty acids Routine analysis Anaerobic fermentation Biogas Process stability Process monitoring |
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