High-performance liquid chromatographic determination of tocopherols in infant formulas |
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Authors: | Rodrigo N Alegría A Barberá R Farré R |
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Institution: | Nutrition and Food Chemistry, Faculty of Pharmacy, University of Valencia, Burjassot, Spain. |
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Abstract: | A method for the simultaneous determination of alpha-tocopherol acetate and alpha-, delta-, and gamma-tocopherols by normal-phase high-performance liquid chromatography (HPLC) with a fluorescent detector in infant formula is proposed. The values obtained in the determination of the analytical parameters: linearity, precision, limit of detection and accuracy (analysis of a standard reference material, SRM 1846), confirm the quality of the method. The proposed method is useful for the determination of alpha-, delta-, and gamma-tocopherols and alpha-tocopherol acetate in infant formulas at a low cost and in a total time of 2 h. |
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