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| 养殖废水中四环素HPLC分析方法的研究 | |
| 引用本文： | 贺蕴普,贺德春,王志良,韩静磊,徐彬.养殖废水中四环素HPLC分析方法的研究[J].广州化学,2011,36(1):26-31. |
| 作者姓名： | 贺蕴普 贺德春 王志良 韩静磊 徐彬 |
| 作者单位： | 1. 中国科学院广州化学研究所,广东广州,510650 2. 湖南农业大学资源环境学院,湖南长沙,410128 3. 江苏省环境科学研究院,江苏南京,210036 4. 环境保护部华南环境科学研究所,广东广州,510655 |
| 基金项目： | 江苏省环境工程重点实验室开放基金资助项目 |
| 摘 要： | 为了评价养殖废水中四环素类抗生素残留的状况,建立了养猪场废水中四种常见四环素类抗生素(土霉素、四环素、金霉素、强力霉素)的高效液相色谱分析方法.水样中四环素类药物经HLB固相萃取小柱吸附,甲醇乙酸乙酯溶液洗脱后用高效液相色谱紫外检测器测定.在0.05～10.00 μg/mL.范围内,四种抗生素的峰面积与质量浓度的线性关... |
| 关 键 词： | 养殖废水 高效液相色谱 四环素 土霉素 金霉素 强力霉素 |
Research of Analytial Meathod about Tetracyclines of Wastewater in Pig Farm by HPLC |
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| HE Yun-pu,HE De-chun,WANG Zhi-liang,HAN Jing-lei,XU Bin.Research of Analytial Meathod about Tetracyclines of Wastewater in Pig Farm by HPLC[J].Guangzhou Chemistry,2011,36(1):26-31. | |
| Authors: | HE Yun-pu HE De-chun WANG Zhi-liang HAN Jing-lei XU Bin |
| Institution: | 1(1.Guangzhou Institute of chemistry of Chinese Academy of Sciences,Guangzhou 510650,China;2.College of Resource and Environment,Hunan Agricultural University,Changsha 410128,China;3.Jiangsu Provincal Academy of Environmental Sciences,Nanjing 210036,China;4.South China Institute of Environmental Sciences,Ministry of Environmental Protection,Guangzhou 510655,China) |
| Abstract: | A method for the determination of 4 tetracyclines(TCs)(oxytetracyline,tetracycline,chlortetracycline,doxytetracyline) redidues in wastewater of pig farm was developed by high performance liquid chromatography(HPLC) with ultraviolet(UV) detector.After adsorbed with a waters oasis hydrophile-lipophile balance solid phase extraction cartridge and eluted by methanol(10%)-acetal,TCs were detected by HPLC-UV.The calibration curve was linear in the range 0.05～10.00 mg/L and R2≥0.999.The limit of quantification(LOQ) was 0.10、0.15、0.30、0.18 μg/L and the recovery rates were 83.35%～93.32% and 86.72%～92.46% with the relative standard deviation(RSD)<8.27% at the spiking level 10.00 and 100.00 ng/mL,respectively,for oxytetracyline,tetracycline,chlortetracycline and doxytetracyline in wastewater.The method was suitable for the detection of TCs residues in wastewater.The four TCs were detected in wastewater sampled from six pig farms. |
| Keywords: | wastewater high performance liquid chromatography(HPLC) tetracycline oxytetracyline chlortetracycline doxytetracyline |
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