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New organoruthenium metallates containing ferrocenecarboxalidine thiosemicarbazones and their nucleic acid/albumin binding and in vitro cytotoxicity |
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| Authors: | Mathiyazhagan Sindhu Palaniappan Kalaivani Rathinasabapathi Prabhakaran |
| Institution: | 1. Department of Chemistry, Nirmala College for Women, Bharathiar University, Coimbatore, 641 018 India
Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization;2. Department of Chemistry, Nirmala College for Women, Bharathiar University, Coimbatore, 641 018 India;3. Department of Chemistry, Bharathiar University, Coimbatore, 641 046 India |
| Abstract: | A string of four new hetero binuclear Ru(III) complexes of ferrocenecarboxaldehyde-4(N)-substituted thiosemicarbazones were synthesized and characterized by various spectral (infrared, ultraviolet–visible, Electron Paramagnetic Resonance (EPR) and High Resolution Mass Spectrometry (HR-MS) techniques. The binding abilities of the ligands/complexes with nucleic acid (calf thymus DNA, CT-DNA) and bovine serum albumin (BSA) were analyzed by absorption and emission titration methods. The complexes exhibited better DNA binding affinity than their parent ligands. The interaction with CT-DNA was found to be intercalative and with BSA static quenching mechanism was observed. All the synthesized Ru(III) complexes were subjected to study their in vitro cytotoxicity against MCF-7 (human breast cancer) and HT-29 (human colon cancer) cell lines. Among the four complexes, complex 3 RuCp (FF-etsc)PPh3]Cl exhibited the highest cytotoxicity in MCF-7 cells and complex 4 RuCp (FF-ptsc)PPh3]Cl was the most active on HT-29 cells. |
| Keywords: | acridine orange-ethidium bromide (AO-EB)/4̕ 6-diamidino-2-phenylindole (DAPI) staining morphological studies MTT assay Ru(III) complexes |