Antibacterial and antioxidant properties of phyto-synthesized silver nanoparticles using Lavandula stoechas extract |
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Authors: | Reza Mahmoudi Saeed Aghaei Zeinab Salehpour Ali Mousavizadeh Seyed Sajjad Khoramrooz Marzie Taheripour Sisakht Gunna Christiansen Marzieh Baneshi Bahman Karimi Hassan Bardania |
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Institution: | 1. Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran;2. Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran;3. Medicinal Plant Research Center, Yasuj University of Medical Sciences, Yasuj, Iran;4. Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran;5. Department of Biomedicine, Aarhus University, Aarhus C, 8000 Denmark;6. Department of Chemistry, Yazd University, Yazd, Iran |
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Abstract: | Due to environmentally friendly and cost- effective issues, biological methods for silver nanoparticles (AgNPs) synthesis are advantageous over chemical and physical ones. In this study, AgNPs synthesized using Lavandula stoechas extract as a reductant and its antioxidant capacity, antibacterial property and cytotoxicity effect were investigated. The phyto-synthesized AgNPs were characterized using various analyses such as transmission electron microscopy (TEM), scanning electron microscopy (SEM), x-ray diffraction (XRD) as well as Fourier transform infrared (FT-IR). The prepared nanoparticles were spherical on shape with the size about 20–50 nm. Antibacterial studies through agar disk diffusion method confirmed the antibacterial potential of phyto-synthesized AgNPs toward two clinical Staphylococus aureus and Pseudomonas aeruginosa bacteria, although MTT assay demonstrated that S. aureus (MIC = 125 μg/ml) was more susceptible to AgNPs than P. aeruginosa (MIC = 250 μg/ml). Moreover, the cytotoxicity assay of phyto-synthezied AgNPs showed a low cytotoxic effect on RAW264 cell line at 62.5 μg/ml as an effective concentration. Also the considerable antioxidant capacity of the AgNPs confirmed through DPPH assay. Great antibacterial and antioxidant properties along with biocompatibility make the suggested phyto-synthesized AgNPs a great candidate for different biomedical applications including wound healing. |
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Keywords: | AgNPs antibacterial activity antioxidant property green synthesis Lavandula stoechas |
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