荧光色谱-柱后光化学衍生法测定食品中的黄曲霉毒素B1

采用免疫亲和层析柱吸附食品样品中的黄曲霉毒素B1，以荧光色谱-柱后光化学衍生法测定食品中黄曲霉毒素B1的含量。样品用甲醇-水溶液（体积比为7：3）混合，均质器高速搅拌提取，经免疫亲和层析柱纯化后，通过LachromC18色谱柱进行分离，以甲醇一水溶液（体积比为45：55）作为流动相，荧光检测器激发波长为360nm、发射波长为420nm，外标法定量。黄曲霉毒素B，的质量浓度在1～20ng／mL范围内与色谱峰面积线性关系良好，相关系数r=0．9998，检出限为0．1gg／kg。在3个添加水平下，样品的加标回收率在88．5％～97．9％之间，相对标准偏差均小于3％（n=6）。该方法操作简单、定量准确，可用于食品中黄曲霉毒素B．的定量定性检验。

Determination ofAflatoxin B1 in Food by Fluorescence Chromatography-Post-Column Photochemical Derivatization Method

Aflatoxin B1 in food sample was adsorbed by using immunoaffinity chromatography column, and the content was determined by fluorescence chromatography-post-column photochemical derivatization method. Samples was mixed with methanol-water（Volume ratio was 7 ： 3）, extracted by homogenizer with high speed stirring, purified by immunoaffinity chromatography column, and separated by Lachrom C18 column with methanol -water （Volume ratio was 45 ： 55）as the mobile phase and fluorescence detector excitation wavelength of 360 nm, emission wavelength of 420 nm, and determined by external standard method. 1-20 ng/mL aflatoxin B1 content and chromatography peak area had a good linear correlation coefficient（r=0.999 8）, the detection limit was 0.1 μ g / kg. In three spiked levels, spiked recoveries were 88.5%-97.9%, the relative standard deviations were less than 3%（n=6）. The improved method is simple, accurate and can be used in food aflatoxin B1 of quantitative and qualitative test.