| 液相色谱串联质谱法测定养殖水体中孔雀石绿及其代谢物 |
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| 引用本文： | 徐彦辉,顾亮,蒋俊树,丁磊.液相色谱串联质谱法测定养殖水体中孔雀石绿及其代谢物[J].化学分析计量,2011,20(2):49-51. |
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| 作者姓名： | 徐彦辉 顾亮 蒋俊树 丁磊 |
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| 作者单位： | 国家农副加工食品质量监督检验中心,安徽国家农业标准化与监测中心,合肥,230051 |
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| 摘 要： | 研究利用液相色谱-串联质谱(LC-ESI-MS/MS)测定水产品养殖水体中孔雀石绿及其代谢物隐性孔雀石绿的方法.通过一系列实验对样品前处理条件进行了优化研究,养殖水样经乙酸酸化,净化后,采用HPLC-ESI-MS/MS检测分析.在多反应监测模式(MRM)下,外标法定量,定量限均为0.5μg/kg.在0.5～50μg/L...
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| 关 键 词： | 养殖水体 孔雀石绿 液相色谱-串联质谱 |
DETERMINATION OF MALACHITE GREEN AND ITS METABOLITES IN AQUATIC WATER BY LIQUID CHROMATOGRAPHY ELECTROSPRAY TANDEM MASS SPECTROMETRY |
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| Xu Yanhui,Gu Liang,Jiang Junshu,Ding Lei.DETERMINATION OF MALACHITE GREEN AND ITS METABOLITES IN AQUATIC WATER BY LIQUID CHROMATOGRAPHY ELECTROSPRAY TANDEM MASS SPECTROMETRY[J].Chemical Analysis And Meterage,2011,20(2):49-51. |
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| Authors: | Xu Yanhui Gu Liang Jiang Junshu Ding Lei |
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| Institution: | ( China National Center For Quality Supervision and Test of Agriculture Avocation Processed Food, National Center of Agricultural Standarlization and Supersion, Hefei 230051 , China) |
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| Abstract: | A new method for determination malachite green and its metabolites in aquatic water by ultra - performance liquid chromatography -electrospray ionization tandem mass spectrometry was developed. Sample preparation and HPLC - MS/MS analytical conditions were examined and optimized critically by a series of experiments. Samples were acidulated by acetic acid ,then cleaned up, and ultimately LC - ESI - MS/MS method was adopted for qualitative and quantitative analysis. Limit of detections （LOD） was 0.5 μg/kg. The method showed good linearity（r 〉 0. 999） at the rang of 0.5 -50 μg/L. The average recoveries ranged from 71.5% to 88.0%. The method is suitable for the trace analysis of MG in aquatic water owing to its high efficiency and convenience, and it can provide a basis for testing standard of malachite green in water. |
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| Keywords: | aquatic water malachite green liquid chromatography -electrospray ionization tandem mass spectrometry |
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