| 毛细管气相色谱法测定深海鱼油中的EPA和DHA含量 |
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| 引用本文： | 黄建立,孙凡,任清.毛细管气相色谱法测定深海鱼油中的EPA和DHA含量[J].化学分析计量,2001,10(5):9-10. |
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| 作者姓名： | 黄建立 孙凡 任清 |
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| 作者单位： | 1. 福建省粮油质量监测所,福州,350002
2. 中科院福建物质结构研究所,福州,350002 |
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| 摘 要： | 用氢氧化钾－甲醇酯化法将深海鱼油中的脂肪酸快速、有效地转化成脂肪酸甲酯，然后进行毛细管气相色谱法测定，以二十二碳饱和脂肪酸甲酯为内标物，建立了5，8，11，14，17－二十碳五烯酸（EPA）和4，7，10，13，16，19－二十二碳六烯酸（DHA）的定量分析方法，并用于实际样品分析。该方法对EPA和DHA定量分析的相对标准偏差分别为4．6％和5．0％，回收率分别为99．7％和99．4％。
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| 关 键 词： | 毛细管气相色谱 深海鱼油 EPA DHA 二十碳五烯酸 二十二碳六烯酸 测定 脂肪酸 |
DETERMINATION OF EPA AND DHA CONTENTS IN DEEP SEA FISH OIL BY CAPILLARY GAS CHROMATOGRAPHY |
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| Huang Jianli,Sun Fan.DETERMINATION OF EPA AND DHA CONTENTS IN DEEP SEA FISH OIL BY CAPILLARY GAS CHROMATOGRAPHY[J].Chemical Analysis And Meterage,2001,10(5):9-10. |
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| Authors: | Huang Jianli Sun Fan |
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| Abstract: | Fatty acids in deep sea fish oils was derived as methyl esters using KOH - CH3OH solution. A quantitative method with C22:0 as internal standard for analyzing eicosapentaenoic acid (EPA) and docosahexaenic acid (DHA) by gas chromatography was established. The precision of the method for the determination of EPA and DHA was 4.6% and 5.0% of RSD, respectively. The recovery of EPA and DHA was 99.7% and 99.4% , respectively. It has been applied to the analysis of actual deep sea fish oil samples. |
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| Keywords: | gas chromatography deep sea fish oil EPA DHA C_(22:0) |
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