HPLC法同时测定乳酸菌饮料中2种防腐剂和3种甜味剂

建立了一种高效液相色谱法同时测定乳酸菌饮料中2种防腐剂（苯甲酸、山梨酸）、3种甜味剂（乙酰磺氨酸钾、天门冬酰苯丙氨酸甲酯、糖精钠）的方法。样品经12％乙酸锌溶液和12％亚铁氰化钾溶液处理后，用C18色谱柱分离-以甲醇和0．02mol／L乙酸铵溶液为流动相梯度淋洗，紫外检测波长为230，205nm，可在30min内将5种组分完全分离，测定结果的相对标准偏差为1．1％-4．3％（n=5），加标回收率为90％-102％。

Simultaneous Determination of Two Preservatives and Three Sweeteners in Lactic Acid Bacteria Beverage by HPLC Method

A rapid and selective HPLC method was developed for the simultaneous analysis of two preservatives and three sweeteners including benzoic acid, sorbic acid, acesulfame potassium （acesulfarne-K）, L-aspartyl-L-phenylalanine methyl ester （aspartame）, saccharin sodium in lactic acid bacteria beverage. The preservatives and sweeteners in samples were extracted with 12% zinc acetate solution and 12% potassium ferrocyanide solution, separated on a C18 column using a mobile phase＂ made up of methanol - 0.02 mol/L ammonium acetate and ultraviolet detection wavelength was 230,205 urn. All the five additives were separated completely in 30 minutes. The recovery was in the range 90% - 102%, and the RSD of determination results was 1.1% - 4.3%（n=5）.