超高效液相色谱法测定美白、祛痘化妆品中4种禁用激素

建立了超高效液相色谱同时测定美白、祛痘化妆品中4种糖皮质激素氢化可的松、曲安奈德醋酸酯、氯倍他索丙酸酯、倍他米松双丙酸酯的方法。样品采用甲醇涡旋超声提取,色谱柱采用Waters ACQUITY UPLC BEH C18柱(100 mm×2.1 mm,1.7μm),流动相为乙腈–水溶液,梯度洗脱,流量为0.5 m L/min,用PDA检测器检测,检测波长为240 nm。方法检出限以3倍空白噪音计,氢化可的松、曲安奈德醋酸酯、氯倍他索丙酸酯、倍他米松双丙酸酯的检出浓度分别为0.12,0.18,0.30,0.30 mg/kg。在质量浓度为1.0~40.0 mg/L时,标准工作曲线相关系数r0.999 8,4种激素的回收率为85.3%~102.8%,测定结果的相对标准偏差为2.1%~5.0%(n=6)。该方法前处理简单,分离效果好,且灵敏度高,速度快,能满足美白、祛痘化妆品中4中糖皮质激素的检测需要。

Determination of Four Prohibited Substances in Whitening or Acne Cosmetics by Ultra-performance Liquid Chromatography

An ultra-performance liquid chromatography method was established for simultaneously determination of four glucocorticoids: hydrocortisone, triamcinolone acetonide acetate, clobetasol 17-propionate and betamethasone dipropionate in whitening or acne cosmetics. After methanol extraction, glucocorticoids were separated on Waters ACQUITY UPLC BEH C18 Column (100 mm×2.1 mm, 1.7μm) by gradient elution using acetonitrile–water as the mobile phase at a flow rate of 0.5 mL/min, and detected by PDA detector at detecting wavelength of 240 nm. The detection limits asS/N=3 were 0.12, 0.18, 0.30, 0.30 mg/kg for hydrocortisone, triamcinolone acetonide acetate, clobetasol 17-propionate andbetamethasone dipropionate, respectively. Within a glucocorticoid concentration range of 1.0–40.0 mg/L, the average recoveries for glucocorticoids were 85.3%–102.8%, the correlation coefficients of linear calibration curve were all more than 0.999 8, the relative standard deviations were 2.1%–5.0%(n=6). The pretreatment is simple and the separation is quick and effective. The method is satisfactory for determination of four glucocorticoids in whitening or acne cosmetics.