| 高效液相色谱法测定武力拔寒散中橙皮苷的含量 |
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| 引用本文： | 赵磊,孙艳涛,张晗,马琳琳,于浪天.高效液相色谱法测定武力拔寒散中橙皮苷的含量[J].化学分析计量,2017,26(3):50-53. |
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| 作者姓名： | 赵磊 孙艳涛 张晗 马琳琳 于浪天 |
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| 作者单位： | 1. 四平市食品药品检验所,吉林四平,136000;2. 吉林师范大学化学学院,吉林四平 136000;吉林师范大学环境友好材料制备与应用省部共建教育部重点实验室,吉林四平 136000;3. 中国医科大学,南京,211198 |
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| 基金项目： | 2016国家级大学创新创业训练计划项目，四平市科技局项目 |
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| 摘 要： | 建立液相色谱测定武力拔寒散中橙皮苷含量的方法。以甲醇为流动相A,以冰乙酸–水(1∶300)为流动相B,进行梯度洗脱,检测波长为284 nm,流量为1.0 m L/min,柱温为30℃。橙皮苷的质量浓度在1.190 3~119.03μg/mL范围与色谱峰面积线性关系良好(r=0.999 9),检出限为300 ng/g。样品加标回收率为95.00%~98.50%,测定结果的相对标准偏差为1.0%(n=6)。该方法简单、快速、准确,重现性好,可为全面评价和控制武力拔寒散制剂的质量提供依据。
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| 关 键 词： | 武力拔寒散 花椒 橙皮苷 高效液相色谱法 |
Determination of Hesperidin in Wuli Bahan Powders by High Performance Liquid Chromatography |
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| Zhao Lei,Sun Yantao,Zhang Han,Ma Linlin,Yu Langtian.Determination of Hesperidin in Wuli Bahan Powders by High Performance Liquid Chromatography[J].Chemical Analysis And Meterage,2017,26(3):50-53. |
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| Authors: | Zhao Lei Sun Yantao Zhang Han Ma Linlin Yu Langtian |
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| Abstract: | A method was established to determine hesperidin in Wuli Bahan powders by high performance liquid chromatography. The mobile phase was methanol (A) and acetic acid-water (1:300)(B) with gradient elution program. The flow rate was 1.0 mL/min and the detection wavelength was 284 nm. The column temperature was 30℃ . The content of hesperidin had good linear relationship with the chromatographic peak area in the range of 1.1903-119.03 μg/mL with the correlation coefficient of 0.9999, the detection limit was 300 ng/g. The sample recovery rates were 95.00％-98.50％, and the RSD was 1.0％(n=6). The method is simple, rapid, accurate and reproducible, which provides a reference for quality control of Wuli Bahan powders. |
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| Keywords: | Wuli Bahan powders chinese prickly ash hesperidin HPLC |
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