火焰原子吸收分光光度法测定维血康颗粒中总铁

建立火焰原子吸收分光光度法测定维血康颗粒中总铁的含量,火焰类型选择空气–乙炔,测定波长为248.3 nm,测定时间为4.0 s,燃气流量为0.9 L/min,以氘灯作为背景校正。铁的质量浓度在2.4~28.8μg/mL范围内与吸光度呈良好的线性,线性相关系数r~2=0.999 8。6次测定结果的相对标准偏差小于1.0%,样品加样回收率为93.89%,方法检出限为0.011 3μg/mL。该方法具有准确度高,稳定性、重复性好等优点,可用于维血康颗粒中总铁含量的测定。

Determination of Total Iron in Weixuekang Granules by Flame Atomic Absorption Spectrometry

A flame atomic absorption spectrometry method was developed for determination of total iron content in weixuekang granules. The air-acetylene was selected asflame type, the detection wavelength was 248.3 nm,determination time was 4.0 s and the gasflow rate was 0.9 L/min. The concentration of iron was linear with absorbance in the range of 2.4-28.8μg/mL, the linear correlation coefficient (r2) was 0.9998. The relative standard deviation of six detection results was less than 1.0%. The standard addition recovery rate was 93.89%, and the detection limit of the method was 0.0113μg/mL. The method had advantages of high accuracy,stability and good reproducibility, and it can be applied to determine the total iron content in weixuekang granules effectively.