HPLC法测定土圞儿根皮和块根中的西瑞香素

建立高效液相色谱法测定土圞儿根皮和块根中西瑞香素含量的方法。采用Kromasil C18色谱柱(250mm×4.6 mm,5μm),以甲醇–0.2%磷酸水溶液(56∶44)为流动相等度洗脱,流量为1.0 m L/min,柱温为35℃,检测波长为345 nm。西瑞香素的质量浓度在20~100μg/m L内与色谱峰面积呈现良好的线性关系,线性相关系数r=0.999 9,方法精密度(RSD)为1.23%(n=6)。根皮样品、块根样品的加标回收率分别为98.8%,98.2%,测定结果的相对标准偏差分别为1.30%,2%(n=6)。该方法快速可靠,可用于土圞儿药材的质量控制。

Determination of Daphnoretin in Root Bark and Roof of Apios Fortunei Maxim by High Performance Liquid Chromatography

The method for determining daphnoretiuin root bark and roof ofapios fortunei maxim by high performance liquid chromatography was established.The separation was performed on Kromasil C18 colunn (250 mm× 4.6 mm,5 μ m)with methanol-0.2％ phosphoric acid aqueous solution (55:44) mobile phase,the flow rate was 1.0 mL/min.The column temperature was 35℃ and the detection wavelength was 345 nm.The mass concentration of daphnoretinin was linear with chromatographic peak area in the range of 20-100 μ g/mL,the correlation coefficient was 0.999 9.The precision of the method (RSD) was 1.23％(n=6).The recovery of daphnoretinin root bark and roof were 98.8％,98.2％,and the relative standard deviation were 1.3％,2％(n=6),respectively.The method is accurate and rapid,and it can be used for the quality control of apios fortunei maxim.