电感耦合等离子体质谱法测定金银花中6种有毒元素

建立电感耦合等离子体质谱法同时测定金银花中铅、镉、铬、镍、铜、砷6种有毒元素含量。采用微波消解法进行前处理,以钪、铟、铋3种元素作为内标物,用电感耦合等离子体质谱法对50批金银花样品中铅、镉、铬、镍、铜、砷6种有毒元素含量进行测定,以内标法定量,并应用SPSS软件对测定值进行统计学分析。6种有毒元素的质量浓度在0~300μg/L范围内线性良好,相关系数均不小于0.9997。6种有毒元素的检出限为0.003~0.020 mg/kg,样品加标回收率为80.0%~111.0%,相对标准偏差为0.71%~3.82%(n=6)。50批金银花样品中共计有14批样品有毒元素含量超出2015年版《中华人民共和国药典》规定,超标率为28%。聚类分析将50批样品分为3大类。该方法操作简单,灵敏度高,专属性好,可准确快速地同时测定金银花中多种有毒元素含量,可作为中药材品质及安全性监管的技术手段。

Determination of six kinds of toxic elements in honeysuckle by ICP–MS

An ICP-MS method was established for simultaneous determination of six kinds of toxic elements including Pb,Cd,Cr,Ni,Cu and As in honeysuckle.50 batches of honeysuckle samples were digested with microwave,and then determined by ICP-MS by using Sc,In and Bi as the internal standard.The determination datas were analyzed using the software of SPSS.The concentrations of six kinds of elements had good linear relations in the range of 0-300μg/L with the correlation coefficients not less than 0.9997.The detection limits were 0.003-0.020 mg/kg,and the recovery rates were 80.0%-111.0%with the relative standard deviations of 0.71%-3.82%(n=6).14 samples of 50 batches of honeysuckle samples had excessive toxic elements content according to Chinese Pharmacopoeia(2015 edition),and the over-standard rate was 28%.The result of cluster analysis showed that the samples could be clustered reasonably into three groups.The method is simple,sensitive and specific for rapid determination of the various toxic elements in honeysuckle,and it will provide references for quality control and safety monitoring of honeysuckle.