金银花不同部位中绿原酸和木犀草苷含量的测定

利用HPLC法测定金银花不同部位中绿原酸和木犀草苷。采用ODS-C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-水(0.03 mol/L磷酸)为流动相进行梯度洗脱,流速:1.0 mL/min,检测波长:350 nm,柱温:室温。绿原酸浓度在0~0.4 mg/mL内与峰面积呈良好线性关系(r2=0.999 6),回收率为101.9%~105.2%,相对标准偏差为3.18%(n=6)。木犀草苷浓度在0~0.1 mg/mL内与峰面积呈良好线性关系(r2=0.999 1),平均回收率为100.5%~104.0%,相对标准偏差为3.42%(n=6)。结果表明:金银花、芽等部位绿原酸和木犀草苷的含量较高。

DETERMINATION OF THE CONCENTRATIONS OF CHLOROGENIC ACID AND LUTEOLOSIDE IN DIFFERENT PARTS OF HONEYSUCKLE

The high performance liquid chromatographic method was developed for determination of chlorogenic acid and lute- oloside in different parts of honeysuckle. ODS- C18 column （250 mm × 4.6 mm, 5μm） was used for gradient elution with a mobile phase of a mixture of methanol - water solution （ containing 0.03 mol/L phosphoric acid） at a flow rate of 1.0 mL/min with the detec- tion wavelength of 350 nm at room temperature. The calibration curve was linear in the range of 0 - 0.4 mg/mL for chlorogenic acid （ r2 = 0. 999 6） , and the recovery of chlorogenic acid was 101.9% - 105.2% with relative standard deviation （RSD） of 3.18% （n = 6）. The calibration curve was linear in the range of 0 - 0.1 mg/mL for luteoloside （ r2 = 0. 999 1 ） , and the recovery of luteoloside was 100.5% - 104.0% with relative standard deviation （RSD） of 3.42% （ n = 6）. The concentrations of chlorogenic acid and luteoloside were higher in flower buds and buds of honeysuckle.