| 丙丁梭菌萃取发酵产物的气相色谱分析 |
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| 引用本文： | 闫海荣,杨丽萍,刘力强,石晨光,贾娟娟,王正品.丙丁梭菌萃取发酵产物的气相色谱分析[J].化学分析计量,2010,19(6):13-16. |
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| 作者姓名： | 闫海荣 杨丽萍 刘力强 石晨光 贾娟娟 王正品 |
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| 作者单位： | 华北制药集团新药研究开发有限责任公司,石家庄050015 |
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| 基金项目： | 河北省科学技术研究与发展计划重大技术创新项目，石家庄市科学应用与技术研究开发资金项目 |
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| 摘 要： | 建立了3种溶剂中产物丙酮、乙醇、正丁醇的气相色谱分析方法。色谱条件为:DB-624色谱柱(30 m×0.32 mm,1.8μm)及FFAP色谱柱(30 m×0.32 mm,0.25μm),FID检测器,程序升温,氮气为载气。采用内标法定量,内标物为异丁醇,进样体积1μL。丙酮、乙醇、正丁醇的浓度与色谱峰面积线性相关,相关系数为0.997 43~0.999 97,测定结果的相对标准偏差为0.19%~2.01%(n=5),回收率为95.1%~101.2%。
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| 关 键 词： | 气相色谱 萃取发酵 丙丁梭菌 |
GC ANALYSIS OF EXTRACTED FERMENTATION PRODUCTS BY CLOSTRIDIUM ACETOBUTYLICUM |
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| Yan Hairong,Yang Liping,Liu Liqiang,Shi Chenguang,Jia Juanjuan,Wang Zhengpin.GC ANALYSIS OF EXTRACTED FERMENTATION PRODUCTS BY CLOSTRIDIUM ACETOBUTYLICUM[J].Chemical Analysis And Meterage,2010,19(6):13-16. |
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| Authors: | Yan Hairong Yang Liping Liu Liqiang Shi Chenguang Jia Juanjuan Wang Zhengpin |
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| Institution: | (North China Pharmaceutical Group Co. New Drug R＆D Co. , Ltd, Shijiazhuang 050015, China) |
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| Abstract: | A new GC analysis method of acetone, butanol, ethanol in different phase was developed. The analytical columns were DB - 624 （ 30 m ×0.32 mm, 1.8 μm） and FFAP （ 30 m × 0.32 mm, 0.25 μm） with a flame ionization detector. The oven temper- ature was programmed, the carrier gas was nitrogen. Internal standard method was used for quantitative analysis and the internal stand- ard was iso-butanol, the injection volume was 1 μL. The concentration of acetone, butanol and ethanol was linear with the peak area, and the linear coefficient was between 0. 997 43 -0. 999 97. The relative standard deviation was 0.19 % -2.01% （ n = 5 ）, and the av- erage recovery was 95.1% -101.2%. |
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| Keywords: | GC extracted fermentation clostridium acetobutylicum |
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