高效液相色谱-紫外检测器法测定化妆品中的苯甲醇

建立了高效液相色谱-紫外检测器法检测化妆品中苯甲醇含量的方法.样品用甲醉超声提取20 min,离心、过滤,用Agilent ZORBAX ODS分析柱分离分析,紫外检测器检测,外标法定量.苯甲醉在0.0464-0.464 mg/mL浓度范围内线性关系良好,相关系数为0.99992.添加水平为46.40 -92.80 m...

DETERMINATION OF BENZYL ALCOHOL IN COSMETICS BY HPLC-VWD

A method was developed for the determination of benzyl alcohol content in cosmetics by HPLC - VWD. The sam- ple was extracted with methanol for 20 min ultrasonicaly. After being centrifugaled and filtrated with a 0.45 ~m film, a Agilent ZORB- AX ODS analytical column was employed for separation and analysis, VWD detector was used to test directly, external standard method were used for quantification. A good linear relationship was obtained in the range of 0.0464 -0. 464 mg/mL with correlation coefficient of 0. 999 92, and recoveries ranged from 82.3% to 118.4% at addition levels of 46.40 - 92.80 mg/L. The relative standard deviation of determination results was 0.14% （n = 6）, and the detection limit （S/N = 3 ） was 0.10 mg/kg. The result showed that this method can meet the detection requirement of benzyl alcohol content in cosmetics.