高效液相色谱-二级管阵列检测器法测定化妆品中邻苯二甲酸酯

用甲醇将化妆品样品超声提取15min，离心、过滤，以XDB-C18分析枉分离分析，二级管阵列检测器检测，结合保留时间和光谱定性分析，采用高效液相色谱法测定化妆品中的邻苯二甲酸酯，外标法定量。邻苯二甲酸酯邻苯二甲酸丁基苄基酯（BBP）、邻苯二甲酸二丁酯（DBP）、邻苯二甲酸二（2-乙基已）酯（DEHP）在0．5-10．0mg／L浓度范围内均有良好的线性关系，相关系数r〉0．9998。BBP，DBP，DEHP在1．000，2．000mg／L添加水平的回收率为92％-105％，相对标准偏差（RSD）小于4％0=6），检出限分别为14，12，6mg／kg。该方法适用于化妆品中BBP，DBP和DEHP含量的检测。

Determination of Phthalates in Cosmetics by HPLC-DAD

The sample was extracted with methanol for 15 rain ultrasoniealy. After being centrifuged and filtrated with 0.45 ~m film, XDB-C18 analytical column was employed for separation and analysis, and DAD detector was used to test directly. External standard method was used for quantification. Linear relationship was found well in the range of 0.5-10.0 mg/L for BBP, DBP and DEHP with correlation coeficient more than 0.999 8. The recoveries ranged from 92% to 105% at addition levels of 1.000,2.000 mg/L for them. The relative standard deviation was less than 4% （n=6）. The detection limit ofBBP, DBP, DEHP were 14, 12,6 mg/kg respectively. The method is suitable for detection ofBBP, DBP and DEHP in cosmetics.