新型表面辅助激光解吸附/离子化质谱基质及其在生物检测中的应用

表面辅助激光解吸附/离子化质谱(Surface-assisted laser desorption/ionization mass spectrometry,SALDI-MS)是一种利用无机纳米粒子或纳米结构表面作为基质,辅助待测分子的解吸附和离子化的质谱技术。由于其具有灵敏度高、耐盐性好、操作简便、重现性好、检测通量高等优势,已经被广泛应用于食品安全、环境监测、生命科学等诸多领域。该文总结了近5年来,SALDI基质材料(金属及金属氧化物材料、碳材料、硅材料、金属有机骨架化合物材料等)的最新研究进展及其在生物检测领域中的应用,并对SALDI-MS基质材料的发展及应用进行了展望。

Novel Matrixes for Surface assisted Laser Desorption/Ionization Mass Spectrometry and Their Applications in Biological Detection

Surface assisted laser desorption/ionization mass spectrometry(SALDI-MS) is a technique using inorganic nanoparticles and nanostructure surface as matrixes to facilitate desorption/ionization of analytes.Due to its advantages of high sensitivity,high salt tolerance,easy operation,good reproducibility and high throughput,SALDI-MS has been widely applied in the fields of food safety,environment monitoring and life science,etc.The recent development of SALDI-MS matrixes such as metal and metal oxide nanoparticles,carbon based materials,silica based materials and metal organic frameworks etc.and their applications in biological detection are summarized in this review.Finally,further perspective for the development of the novel matrixes and their potential applications are proposed.