MALDI质谱技术在酶活性分析中的应用

酶作为生物催化剂参与很多重要的生理过程,同时也是一类重要的生物分子。酶的活性分析对于疾病诊断和治疗具有重要意义。基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)具有操作简单、分析速度快、灵敏度高和易于实现高通量分析的特点,已被广泛用于各种组学研究和生物分子的检测,在酶的检测和活性分析中亦发挥了重要作用。该文综述了国内外利用MALDI-TOF MS分析酶活性和进行药物筛选的策略,总结了各种方法的优缺点,提出了MALDI质谱技术在酶活性分析领域存在的问题和挑战,并对其发展前景进行了展望。

Application of MALDI Mass Spectrometry in Enzymes Activity Analysis

Enzymes as biocatalysts,participate in many physiological processes,which are important biomarkers.The activity analysis of enzymes is of great significance for diagnosis and treatment of many diseases.Matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS) has been widely used in various omics studies and biological molecules detections because of its good performances in sensitivity,throughput,speed and operation.It also has played an important role in enzymes activity analysis.The methods of MALDI-TOF MS for enzyme activity analysis and drug screening are reviewed in this paper,the merits and disadvantages of each method are discussed,the problems and challenges existed are put forward,and finally the perspectives for MALDI-TOF MS in enzyme activity analysis are outlooked.