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密闭微波辅助提取-HPLC法测定还亮草中硬飞燕草碱和巴比翠雀碱的含量
引用本文:樊轻亚,郝艳红,代春美.密闭微波辅助提取-HPLC法测定还亮草中硬飞燕草碱和巴比翠雀碱的含量[J].分析测试学报,2017,36(1):106-111.
作者姓名:樊轻亚  郝艳红  代春美
作者单位:1. 信阳职业技术学院药学院,河南信阳,464000;2. 信阳职业技术学院医学院,河南信阳,464000;3. 中国中医科学院,北京,100700
基金项目:第24批中国博士后科技服务团合作项目
摘    要:采用密闭微波辅助法(PMAE)提取还亮草中的硬飞燕草碱和巴比翠雀碱。采用单因素试验结合正交试验方法对微波实验条件进行优化。得到硬飞燕草碱和巴比翠雀碱的最佳提取方案:药物颗粒度100目,固液比1∶60,微波温度80℃,微波功率560 W,微波时间10 min。以甲醇-0.2%三乙胺(45∶55)为流动相,建立了高效液相色谱(HPLC)测定硬飞燕草碱和巴比翠雀碱含量的方法。硬飞燕草碱和巴比翠雀碱分别在0.50~50.0,0.30~30.0 mg/L范围内呈良好的线性关系,回收率为98.3%~104.5%,相对标准偏差(RSD)分别为2.0%和2.2%。与传统溶剂回流法(SRE)进行比较,该方法简单、提取率更高。

关 键 词:还亮草  密闭微波提取  硬飞燕草碱  巴比翠雀碱  正交试验

Determination of Belsoline and Eldeline Contituents in Delphinium Anthriscifolium Hance by Microwave-assisted Extraction with HPLC
FAN Qing-y,HAO Yan-hong,DAI Chun-mei.Determination of Belsoline and Eldeline Contituents in Delphinium Anthriscifolium Hance by Microwave-assisted Extraction with HPLC[J].Journal of Instrumental Analysis,2017,36(1):106-111.
Authors:FAN Qing-y  HAO Yan-hong  DAI Chun-mei
Abstract:A method of extraction of belsoline and eldeline in Delphinium anthriscifolium Hance was developed by pressurized microwave-assisted extraction (PMAE).The single factor test and orthogonal test were used to optimize the experimental conditions.The optimum extraction conditions were as follows:particle size:100 mesh,ratio of solid to liquid:1 ∶ 60,microwave temperature:80 ℃,microwave power:560 W,microwave time:10 min.Under the optimal conditions,belsoline and eldeline were separated,and determined by HPLC using methanol-0.2% triethylamine (45 ∶ 55) as mobile phase.The linear ranges of belsoline and eldeline were in the ranges of 0.50-50.0 mg/L and 0.30-30.0 mg/L,respectively.The recoveries of belsoline and eldeline were in the range of 98.3%-104.5%,and RSDs were 2.0% and 2.2%,respectively.Compared with the solvent reflux extraction method (SRE),the proposed method was simple,and showed high extraction efficiencies.
Keywords:Delphinium anthriscifolium Hance  pressurized microwave-assisted extraction  belsoline  eldeline  orthogonal test
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