纳米金-还原氧化石墨烯修饰葡萄糖氧化酶传感器的制备及其电流法检测饮料中的葡萄糖

利用纳米金(Au NPs)与还原氧化石墨烯(rGO)复合纳米材料制备了葡萄糖氧化酶生物传感器并用于饮料中葡萄糖含量的检测。将壳聚糖作为还原剂及稳定剂,通过一步法合成了Au NPs-rGO复合材料,并通过物理吸附固定葡萄糖氧化酶(GOx)来制作GOx生物传感器。该传感器在磷酸盐缓冲溶液(0.1 mol/L,p H6.0)中,-0.45 V(vs.Ag/Ag Cl)电位下电流法检测葡萄糖含量,线性检测范围为0.01~0.88 mmol/L,灵敏度为22.54μA·mmol-1·L·cm-2,检出限为1.01μmol/L,且表观米氏常数为0.497 mmol/L。该传感器用于多种饮料中葡萄糖含量的直接检测,结果满意。

Preparation of a Gold Nanoparticles-Reduced Graphene Oxide Modified Glucose Oxidase Biosensor and Its Amperometric Determination of Glucose in Beverages

An amperometric glucose oxidase (GOx) biosensor based on gold nanoparticles (AuNPs) -reduced graphene oxide (rGO) was fabricated for the detection of glucose in beverages.AuNPs-rGO composite was synthesized by one-pot method with chitosan as reducing and stabilizing agent.It was used as the matrix for GOx immobilization via physical adsorption technique to fabricate the GOx-based biosensor.The biosensor was applied in the amperometric determination of glucose at-0.45 V(vs.Ag/AgCl) in a phosphate buffer (0.1 mol/L,pH 6.0).There was a linear response to glucose in the concentration range of 0.01-0.88 mmol/L,with a sensitivity of 22.54 μA · mmo1-1.L · cm-2 and a detection limit of 1.01 μmol/L.The apparent Michaelis-Menten constant was rather small(0.497 mmol/L).The constructed biosensor was successfully applied in the detection of glucose in beverages.