| 土霉素的电化学性质及其与DNA相互作用的研究 |
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| 引用本文： | 张敏,程发良,姚海军.土霉素的电化学性质及其与DNA相互作用的研究[J].分析测试学报,2007,26(5):634-637. |
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| 作者姓名： | 张敏 程发良 姚海军 |
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| 作者单位： | 东莞理工学院,生物传感器研究中心,广东,东莞,523106 |
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| 基金项目： | 国家自然科学基金资助项目(20375008,20475001)，广东省科技攻关项目(2004B33301024,2005B10301041,2006B12401011)，广东省自然科学基金资助项目(06108856) |
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| 摘 要： | 研究了土霉素在玻碳电极上的电化学行为。并利用电化学方法研究了土霉素与小牛胸腺DNA(ctDNA)的相互作用,DNA的存在能导致土霉素还原峰电流降低,峰电位正移,推测土霉素与DNA在该条件下以键合模式相互结合。紫外-可见吸收光谱的研究进一步确证了上述结果。
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| 关 键 词： | 土霉素 DNA修饰电极 循环伏安法 嵌插作用 紫外-可见光谱 |
| 文章编号： | 1004-4957（2007）05-0634-04 |
| 收稿时间： | 2006-07-17 |
| 修稿时间： | 2006-10-08 |
Electrochemical Investigation on Interaction between Oxytetracyclin and DNA |
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| ZHANG Min,CHENG Fa-liang,YAO Hai-jun.Electrochemical Investigation on Interaction between Oxytetracyclin and DNA[J].Journal of Instrumental Analysis,2007,26(5):634-637. |
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| Authors: | ZHANG Min CHENG Fa-liang YAO Hai-jun |
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| Institution: | Research Institute of Bio-sensor;Dongguan University of Technology;Dongguan 523106;China |
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| Abstract: | The DNA modified glassy carbon electrode prepared using "air-drying/adsorption" method is characterized by means of cyclic voltammetry(CV) and it shows good stability.The electrochemical behaviors of oxytetracyclin are probed by CV.The interaction of oxytetracyclin with calf thymus DNA is also studied,and the interactive mode between oxytetracyclin and DNA is verified by the fluorescence method.The results of sample determined by electrochemical method are consistent with those done by UV-Vis spectrophotometry. |
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| Keywords: | Oxytetracyclin DNA modified glassy carbon electrode Cyclic voltammetry Intercalation binding UV-Vis spectrophotometry |
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